Biostatistics
- Measures describing the central tendency distributions- average, median, mode
- Measures of dispersion: Dispersion, Range, standard deviation
- Probability: Normal distribution and properties.
- Parametric test: t-test (Sample, Pooled or Unpaired and Paired), ANOVA, (One way and Two way).
- Correlation: Definition, Karl Pearson’s coefficient of correlation.
- Regression modeling: Hypothesis testing in Simple and Multiple regression models
- Graphs: Histogram, Pie Chart, Cubic Graph.
- Statistical Analysis Using Excel, SPSS, Graph pad prism.
- Basic fundamental of molecular biology used in bioinformatics
Fundamental of bioinformatics
- Basic fundamental of molecular biology used in bioinformatics
- Biological Databases:Nucleotide/ Genome Databases (GenBank, EMBL, DDBJ) , Protein Sequence Database (Uniprot-KB: SWISS-PROT), Structure databases (PDB, NDB, PubChem). Sequence file formats: Various file formats for bio-molecular sequences: GenBank, FASTA, GCG, MSF etc.
- Sequence Analysis – Basic concepts of sequence similarity, identity and homology, definitions of homologues, orthologues, paralogues and xenologues
- Scoring matrices, PAM and BLOSUM, Sequence alignment – Measurement of sequence similarity; Similarity and homology. Pairwise sequence alignment: Basic concepts of sequence alignment, use of pairwise alignments for analysis of Nucleic acid and protein sequences and interpretation of results.
Basic tools and popular software in bioinformatics
- Primer designing for PCR, RT PCR, Molecular Marker
- Pairwise alignment and Multiple sequence alignment; ClustalW
- Phylogenetic analysis; MEGA
- Dot Plot
- Use of in sequence manipulation Bioedit.
- Motif analysis: MEME
